org.springframework.http.converter.HttpMessageNotReadableException: JSON parse error: Unexpected character (‘ï’ (code 239)): was expecting a colon com.fasterxml.jackson.core.JsonParseException: Unexpected character (‘ï’ (code 239)): was expecting a colon
从Gene Expression Omnibus数据中收集5个结肠癌样本微阵列数据和癌症基因组图谱(TCGA)。在预处理之后,GSE17537中的数据是使用用于微阵列数据的线性模型(LIMMA)方法鉴定差异表达基因(DEGs)。 DEG进一步进行了基于PPI网络的社区评分和支持向量机(SVM)。然后使用SVM和Cox回归分析通过四个数据集GSE38832,GSE17538,GSE28814和TCGA验证。
colon$status.factor <- factor(colon$status,labels=c("Alive","Died")) colon$obstruct.factor <- factor( colon$obstruct,labels=c("No","Yes")) colon$perfor.factor <- factor(colon$perfor,labels=c("No","Yes")) colon$status.factor <- setLabel(colon$status.factor,"Mortality") colon$rx <- setLabel(colon$rx,"Treatment ") colon$age <- setLabel(colon$age,"Age(Years)") colon$sex.factor <- setLabel(colon$sex.factor,"Sex") (colon$perfor.factor,"Perforation") colon$nodes <- setLabel(colon$nodes,"Positive nodes") fit <- glm
_16S7255675', 'feature_types-Human_colon_16S7255675', 'gene_ids-Human_colon_16S7255676', 'feature_types-Human_colon _16S7255676', 'gene_ids-Human_colon_16S7255677', 'feature_types-Human_colon_16S7255677', 'gene_ids-Human_colon _16S7255678', 'feature_types-Human_colon_16S7255678', 'gene_ids-Human_colon_16S7255679', 'feature_types-Human_colon _16S7255679', 'gene_ids-Human_colon_16S7255680', 'feature_types-Human_colon_16S7255680', 'gene_ids-Human_colon _16S7255682', 'gene_ids-Human_colon_16S8001863', 'feature_types-Human_colon_16S8001863', 'gene_ids-Human_colon
colon_pathwayCollection是一个典型的通路基因子集的例子。 包括两种元素:一个列表(colon_pathwayCollectionpathways)是15个通路及其对应的通路中基因;一个(colon_pathwayCollectionTERMS)是15个通路的名字 ") CreatePathwayCollection( sets_ls = colon_pathwayCollection$pathways, TERMS = colon_pathwayCollection " ) 6. getPathpVals #计算有监督PCA的通路P值 colon_superpc <- SuperPCA_pVals( colon_Omics, numPCs = 2, # ) getAssay(colon_Omics) getPathwayCollection(colon_Omics) (2)SubsetOmicsResponse 从OmicsSurv中提取response_numh
::::::::::::::::::::::::::::::::::::::::::::::::::: fit <- survfit(Surv(time, status) ~ sex, data = colon ) ggsurvplot_facet(fit, colon, facet.by = "rx", palette = "jco", pval = TRUE) ? variables: rx and adhere #:::::::::::::::::::::::::::::::::::::::::::::::::::::::: ggsurvplot_facet(fit, colon ::::::::::::::::::::::::::::::::::::::::::::: fit2 <- survfit(Surv(time, status) ~ sex + rx, data = colon ) ggsurvplot_facet(fit2, colon, facet.by = "adhere", palette = "jco", pval = TRUE) ?
, Left Side colon cancer GSM6048348 Patient 3, Tumor, Right Side colon cancer GSM6048349 Patient 4, Tumor , Left Side colon cancer GSM6048350 Patient 5, Tumor, Right Side colon cancer GSM6048351 Patient 6, Tumor , Right Side colon cancer GSM6048354 Patient 9, Tumor, Left Side colon cancer GSM6048355 Patient 10, Tumor, Left Side colon cancer GSM6048356 Patient 11, Tumor, Right Side colon cancer GSM6048357 Patient 12, Tumor, Right Side colon cancer GSM6048358 Patient 13, Tumor, Right Side colon cancer GSM6048359
# Output Files wget https://cf.10xgenomics.com/samples/spatial-exp/3.0.0/Visium_HD_Human_Colon_Cancer_P1 /3.0.0/Visium_HD_Human_Colon_Cancer_P1/Visium_HD_Human_Colon_Cancer_P1_spatial.tar.gz wget https://cf .10xgenomics.com/samples/spatial-exp/3.0.0/Visium_HD_Human_Colon_Cancer_P1/Visium_HD_Human_Colon_Cancer_P1 /3.0.0/Visium_HD_Human_Colon_Cancer_P1/Visium_HD_Human_Colon_Cancer_P1_feature_slice.h5 wget https:// cf.10xgenomics.com/samples/spatial-exp/3.0.0/Visium_HD_Human_Colon_Cancer_P1/Visium_HD_Human_Colon_Cancer_P1
gtid_set = GTID_NORMALIZE(gtid_set); SET colon_pos = LOCATE2(':', gtid_set, 1); WHILE colon_pos ! = LENGTH(gtid_set) + 1 DO SET next_dash_pos = LOCATE2('-', gtid_set, colon_pos + 1); SET next_colon_pos = LOCATE2(':', gtid_set, colon_pos + 1); SET next_comma_pos = LOCATE2(',', gtid_set, colon_pos ) - (next_dash_pos + 1)) - SUBSTR(gtid_set, colon_pos + 1, next_dash_pos - (colon_pos + 1)) + 1; ELSE SET result = result + 1; END IF; SET colon_pos = next_colon_pos; END
"Colon adjacent normal 466T", "Colon cancer tissue 466T", "Colon adjacent normal 469T", "Colon cancer tissue 469T", "Colon adjacent normal 398T", "Colon cancer tissue 398T", "Colon adjacent normal 361T", "Colon cancer tissue 361T", "Colon cancer tissue 467T", "Colon adjacent normal 467T") ) 我们需要把IDAT的文件名字加到
(fit2, colon, facet.by = "adhere", palette = "jco", pval = TRUE) 同时绘制多个生存函数 data(colon ) ## Warning in data(colon): data set 'colon' not found f1 <- survfit(Surv(time, status) ~ adhere, data = colon) f2 <- survfit(Surv(time, status) ~ rx, data = colon) fits <- list(sex = f1, rx = f2) # = colon$status, pfs.time = sample(colon$time), pfs.status = colon$status, sex = colon$sex, rx = colon$rx, adhere = colon$adhere ) # 总体的PFS和OS生存曲线 pfs <- survfit( Surv(pfs.time, pfs.status) ~
: Some( Colon, ), // 字段类型 String qself: None, path: Path { leading_colon 属性名称 builder path: Path { leading_colon qself: None, path: Path { leading_colon , ), // Meta meta: Meta::List { path: Path { leading_colon
绘制简单生存曲线 使用colon数据集进行演示。 data(colon) # 加载内置数据集 fit <- survfit(Surv(time,status) ~ sex, data = colon) # 拟合生存曲线 ggsurvplot(fit, data = colon) # 绘制大图 ? ggsurvplot(fit, data = colon, ylim = c(0.4, 1)) # 绘制小图 ? install.packages("eoffice") # 安装包 library(eoffice) # 加载包 # 导出大图 ggsurvplot(fit, data = colon) # 绘制图形
的数据挖掘文章:《Molecular Pathway Analysis Indicates a Distinct Metabolic Phenotype in Women With Right-Sided Colon Cancer》,针对4个公共的表达量芯片数据集,分别是:SE41258, GSE39582, GSE37892, and GSE14333,做差异分析,这样的话样品数量就很可观了 : right-sided colon cancers (RCCs) (tumors arising between the cecum and proximal transverse colon) left-sided colon cancers (LCCs) (tumors arising between the distal transverse colon and sigmoid colon, excluding the rectum). GSE14333数据集里面,就有记录CRC病人的Left 和 Right 信息,可以做差异分析,如下所示的病人临床信息 > table(group_list) group_list Colon
) ggsurvplot(fit, data = colon, fun = "cumhaz", #定义生存曲线变换的任意函数,"event"累积事件f(y) = 1-y, "cumhaz"累积风险函数 ) fit2 <- surv_fit(Surv(time, status) ~ adhere, data = colon) fit.list <- list(sex = fit1, adhere = fit2 ) ggforest(model) #Fig 19 colon <- within(colon, {sex <- factor(sex, labels = c("female", "male")) ", "muscle", "serosa", "contig.")) }) head(colon)#Fig 20 bigmodel<-coxph(Surv(time, status) ~ sex + rx + adhere + differ + extent + node4,data = colon ) ggforest(bigmodel, fontsize=1.5,#图中注释的相对大小,默认0.7
3,826 (ST-P1, liver), 4,658 (ST-P2, liver), 3,695 (ST-P3, liver), 3,721 (ST-P4, liver), 3,313 (ST-P1, colon ), 4,174 (ST-P2, colon), 4,007 (ST-P3, colon), and 3,902 (ST-P4, colon) spots. $`ST-colon2` An object of class Seurat 41100 features across 4174 samples within 2 assays Active $`ST-colon3` An object of class Seurat 38937 features across 4007 samples within 2 assays Active $`ST-colon4` An object of class Seurat 39968 features across 3902 samples within 2 assays Active
1.html 本研究纳入的病人队列 在文章附件有详细描述: Patient ID Gender Age AJCC Stage Primary Site CRC01 Female 50 IV Left Colon CRC02 Female 56 IV Right Colon CRC03 Male 60 III Rectum CRC04 Female 71 III Right Colon CRC06 Female 59 III Left Colon CRC09 Male 71 IV Left Colon CRC10 Male 63 III Left Colon CRC11 Female 51 III Left Colon CRC12 Female 49 III Rectum CRC13 Male 51 III Left Colon CRC14 Female 76 III Rectum CRC15 Female 79 IV Right Colon 可以看到实验设计还是蛮周全的,每个病人在不同治疗时间点,都取样了,而且每个样品都使用了scTrio-seq技术进行探索。
''} ); for ii=1:numel( txt ) if isempty( txt{ii} ) continue; end % Look for the colon that separates the property name from the value colon = find( txt{ii}==':', 1, 'first' ); if isempty( colon ) || colon==1 || colon==length( txt{ii} ) continue; end fieldName = strtrim ( txt{ii}(1:colon-1) ); fieldValue = strtrim( txt{ii}(colon+1:end) ); if isempty( fieldName )
包括共定位等等)实现方法,以10X数据为例###下载数据curl -O https://cf.10xgenomics.com/samples/spatial-exp/3.0.0/Visium_HD_Human_Colon_Cancer /Visium_HD_Human_Colon_Cancer_tissue_image.btf####Visium HD output filecurl -O https://cf.10xgenomics.com /samples/spatial-exp/3.0.0/Visium_HD_Human_Colon_Cancer/Visium_HD_Human_Colon_Cancer_binned_outputs.tar.gztar -xvzf Visium_HD_Human_Colon_Cancer_binned_outputs.tar.gz.└── binned_outputs/ └── square_002um/ 1000 # Number of highly variable genes to use n_clusters: 4 chunks_to_run: []cell_markers: # Human Colon
currentToken = { type: 'key', value: '' } return onKey } if (currentToken.type === 'colon return onAarry } } function onColon(e) { if (colonReg.test(e)) { currentToken = { type: 'colon -----// [// { type: 'objectStart', value: '{' },// { type: 'key', value: 'name' },// { type: 'colon ', value: ']' },// { type: 'comma', value: ',' },// { type: 'key', value: 'son' },// { type: 'colon :' },// { type: 'objectStart', value: '{' },// { type: 'key', value: 'nickname' },// { type: 'colon